The flow cytometry separators FACSAriaII and FACSVantage allow cells to be separated in function of parameters previously defined by the operator. To purify different cell populations the flow cytometer is specially designed for applications that require a very pure collection. The simultaneous use of multiple fluorochromes allows subpopulations to be classified and separated in function of different cell properties.
The autoMACS (Miltenyi Biotec) system permits automatic cell separation using paramagnetic beads attached to the cell surface by monoclonal antibodies. It can separate up to 4 x 109 cells in 5 minutes and carry out different separation processes sequentially enriching the subpopulation up to 10,000 times. The preparation of the cell separation experiment requires a series of steps to be carried out previously and this must be agreed with the personnel of the platform ahead of time.
Acquisition and analysis with flow cytometer analysers
FACSCantoII, FACSVerse and LSRFortessa are the cytometer analysers that the user can use to carry out research. These facilities can be used directly by the user if he or she can prove experience in handling the machines or has had previous training from the Cytometry staff. Additionally, the cell separator FACSAria II can be used as a a cytometer analyser.
Off-line analysis with FACSDiva and FlowJo software with offline compensation with off-line scales "Logicle" or biexponenciales; CellQuestPro, Paint-a-Gate and ModFit.
Design of personalized courses. Annually several courses are carried out in the IGTP on the fundamentals and applications of flow cytometry. See our training section.
The Flow Cytometry Unit carries out a consulting service for users on matters connected with flow cytometry.