Microscopy

Abberior Infinity super-resolution microscope

This microscope is housed at the Comparative Medicine and Bioimage Centre of Catalonia (CMCiB) NCB3 facility.

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Abberior STEDYCON

Abberior STEDYCON is a compact confocal & super-resolution microscope capable of 4-colour imaging in confocal and STED super resolution modes (resolution down to 40 nm). STEDYCON is coupled to fully motorised Olympus IX83 body equipped with QUAD-band epifluorescence illumination as well as 10x, 20x dry objectives plus 40x and 63x oil objectives. STEDYCON software for obtaining and analysing images also comes with SVI Huygens Pro software for fast image stitching and deconvolution.

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Leica DMI6000 B inverted microscope

Fully motorised Leica DMI6000 B inverted widefield microscope for brightfield and fluorescence imaging with fluorescence filter sets for UV, Blue and Orange light excited fluorophores as well as 4x, 10x, 20x, 40x dry objectives plus 63x oil objective. The microscope is coupled to Leica DFC420 5MP colour and DFC360 1.4 MP monochrome camera, and utilises Leica LAS X software for obtaining and analysing images. 

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Zeiss Axio Observer Z1 inverted microscope

Zeiss Axio Observer Z1 inverted widefield microscope for brightfield and epifluorescence imaging with fluorescence filter sets for UV, Blue and Orange light excited fluorophores as well as 10x, 20x, 40x dry objectives plus 40x and 63x oil objectives. The microscope is coupled to Zeiss AxioCam MRm 1.4 MP monochrome and utilises ZEN blue 2012 software for obtaining and analysing images. For live cell imaging studies, the microscope is equipped with an incubation chamber (full enclosure) with a modular heating unit for Petri dishes, microscope slides, and 24-well plates and regulation of temperature and CO2 environment.

Zeiss LSM 710 confocal module

Zeiss LSM 710 confocal module coupled to Zeiss Axio Observer Z1 microscope equipped with 7 laser beams (405nm, 458nm 488nm 514nm 543nm, 594nm, 633nm) as well as 10x, 20x, 40x dry objectives plus 40x oil and 63x oil objectives and ZEN black 2012 software for obtaining and analysing images.

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Zeiss PALM

Zeiss PALM microdissection system for non-contact sampling and capture of RNA, DNA and proteins as well as live single cell cloning and culture. This fully motorised system built on Zeiss Axio Observer Z1 inverted microscope is equipped 10x, 20x, 40x dry objectives for brightfield-only imaging and microdissection. Laser Microdissection and Pressure Catapulting (LPMC) is realised via 355 nm UV laser. The microscope is coupled to Hitachi HV-D30 and Zeiss AxioCam MRc5 5 MP colour cameras.

Leica CM1950 cryostat

Leica CM1950 cryostatic microtome with UVC disinfection and Ag-protect system for the manipulation of potentially dangerous specimens. The cryostat is semi-automatic with 15 plate positions, working temperature range from 18 °C to -42 °C, rapid freezing of samples to -52 °C and vacuum washing system to reduce direct manipulation of samples.

Leica M80 stereo microscope

Leica M80 stereo microscope for microdissections equipped with LED3000 RL ring illuminator, transmitted light base as well as KL1500 LCD spotlight illumination light source. The microscope is coupled to a stand-alone digital imaging system based on FLEXCAM C1 12 MP colour camera.