Cytometry

Cell Sorters

The FACSAria II (BD Biociencias) cell separator

Three lasers (405, 488 y 633 nm), 13 fluorescent detectors (13 fluorescent and 2 light scattering). ACDU (Automated Cell Deposition Unit) for sorting different types of well plates and slides. Options installed: aerosol evacuation system (AMO) and refrigerated bath temperature control. Software FACSDiva. Sorting speed >20.000 cells/sec.

Flow Cytometer Analysers

Flow Cytometer LSRFortessa SORP (BD Biociencias)

Equipped with solid state blue laser (488nm), red laser He-Ne (640 nm), violet laser (405 nm) and green laser (532 nm) this machine allows you to work with 18 fluoresences and 2 scatter parameter (FSC and SSC) analysing area, height and width of pulse for each one. Digital system processing system with software FACSDiva. HTS system for acquiring samples in 96 well plates.

Flow Cytometer FACSCanto II (BD Biociencias)

Solid state blue laser (488 nm), red He-Ne laser (633 nm) and violet laser (405 nm). This machine can work with eight fluorescences and two dispersion parameters (FSC and SSC) analysing area, height and width. Digital system processing system with FACSDiva software. This program permits off-line compensation and the configuration of biexponential scales.

Flow Cytometer FACSLyric (BD Biosciences)

Equipped with Solid state red He-Ne laser (633 nm) and violet laser (405 nm), this machine collects 10 parameters (FSC, SSC and 8 fluorescences¡ channels). It has an automatic loader for tubes and plates. It includes volume computation and uses FACSuite software.

Luminex® 200 (Luminex Corp.)

Luminex® 200 System is a flexible analyzer based on the principles of flow cytometry. Luminex® 200 sets the standard for multiplexing and offers the ability to perform up to 100 different tests in a single reaction volume and use either magnetic beads or plain polystyrene beads. The equipment performs higher sample throughput with a 96-well plate read time of only 45 minutes.

Off-line analysis software

Software

FlowJo, BD FACSDiva and CellQuestPro are installed in Apple iMac Intel i7 16 GB RAM with MAC OSX and Windows (XP y 7) systems.

High-Dimensional Spectral Cytometry

Aurora 5-laser (Cytek Biosciences)

Configured with five lasers (355, 405, 488, 561 and 640 nm), three scatter parameters and 64 fluorescence channels, Aurora allows high dimensional data with designed panels (40-colors simultaneously and beyond). Equipped with Automatic Micro-sampling System (AMS) for high throughput acquisition with 96-well plates. Data analysis with SpectroFlo software for unmixing spectral raw data.