Proteomics and Metabolomics

Proteomics Services

Identification

  • Identification/simple sample analysis ESI-QqTof MS/MS (BrukerMicrOTOFQ) MASCOT
  • Identification Protein /simple sample analysis MALDI MS/MS (BrukerMicrOTOFQ)

Protein identification in complex samples (Shotgun proteomics)

  • Identification in complex nano HPLC samples ESI-QqTof MS/MS (BrukerMicrOTOFQ) MASCOT
  • Identification by De Novo sequencing HPLC ESI-QqTof MS/MS

Discovery peptide profiles

  • LC/MS profile nanoHPLC sample ESI-QqTof MS (Agilent 1200 BrukerMicrOTOFQ) (ProfileAnalysis)
  • Clinical proteomic MS ClinProt profiles and statistics (ClinProtTools)

Targeted peptide profiles

  • LC/MS profile nanoHPLC sample ESI-QqTof MS (Agilent 1200 BrukerMicrOTOFQ)
  • uPLC ESI-QQQ Analysis, MRM (Agilent 1260 Agilent 6410BA)

Absolute and relative quantification

  • Isotopic labelling
  • Label free
  • Post-translation modification analysis
  • Characterization of immunoprecipitated proteins
  • Purification of recombinant proteins and antibodies using low pressure chromatography
  • FPLC Äktabasic with combined column, affinity, high-performance exclusion, ion-exchange and reverse phase strategies
  • Characterization of intact human recombinant proteins T3 MS
  • MALDI Imaging

Metabolomics Services

Profiles of metabolites of all types (volatile, semi-volatile and non-volatile, both polar and non-polar) generally for biomarker discovery        

  • GC/MS Analysis (Thermo GC8060 MD800 EI+, CI-, CI+)
  • LC/MS profile nanoHPLC sample ESI-QqTof MS (Agilent 1200 BrukerMicrOTOFQ) (ProfileAnalysis)
  • uPLC ESI-QQQ Analysis (Agilent 1260 Agilent 6410BA)

Identification and description of metabolites

  • GC/MS analysis (Thermo GC8060 MD800 EI+)
  • LC/MS/MS analysis nanoHPLC sample ESI-QqTof MS
  • Absolute and relative quantification of small molecules
  • GC/MS SIM analysis (Thermo GC8060 MD800 EI+, CI-, CI+)
  • GC FID analysis (Perkin Elmer)
  • LC/MS profile nanoHPLC sample ESI-QqTof MS (Agilent 1200 BrukerMicrOTOFQ) (ProfileAnalysis)
  • uPLC analysis ESI-QQQ , MRM (Agilent 1260 Agilent 6410BA)

Within this section our unit has developed procedures for:

  • medium and large short-chain fatty acids in serum and cell membranes
  • amino acids
  • organic acids in urine
  • acylcarnitines in plasma
  • prostanoids in plasma
  • volatile carbon compounds in breath
  • free plasmatic thyroid hormones
  • steroid hormones in saliva
  • sugars in urine
  • mucopolysaccharides in serum and urine