Proteomics and Metabolomics

Proteomics Services

Identification

  • Identification/simple sample analysis ESI-QqTof MS/MS (BrukerMicrOTOFQ) MASCOT
  • Identification Protein /simple sample analysis MALDI MS/MS (BrukerMicrOTOFQ)

Protein identification in complex samples (Shotgun proteomics)

  • Identification in complex nano HPLC samples ESI-QqTof MS/MS (BrukerMicrOTOFQ) MASCOT
  • Identification by De Novo sequencing HPLC ESI-QqTof MS/MS

Discovery peptide profiles

  • LC/MS profile nanoHPLC sample ESI-QqTof MS (Agilent 1200 BrukerMicrOTOFQ) (ProfileAnalysis)
  • Clinical proteomic MS ClinProt profiles and statistics (ClinProtTools)

Targeted peptide profiles

  • LC/MS profile nanoHPLC sample ESI-QqTof MS (Agilent 1200 BrukerMicrOTOFQ)
  • uPLC ESI-QQQ Analysis, MRM (Agilent 1260 Agilent 6410BA)

Absolute and relative quantification

  • Isotopic labelling
  • Label free
  • Post-translation modification analysis
  • Characterization of immunoprecipitated proteins
  • Purification of recombinant proteins and antibodies using low pressure chromatography
  • FPLC Äktabasic with combined column, affinity, high-performance exclusion, ion-exchange and reverse phase strategies
  • Characterization of intact human recombinant proteins T3 MS
  • MALDI Imaging