Proteomics and Metabolomics

Metabolomics Services

Profiles of metabolites of all types (volatile, semi-volatile and non-volatile, both polar and non-polar) generally for biomarker discovery        

  • GC/MS Analysis (Thermo GC8060 MD800 EI+, CI-, CI+)
  • LC/MS profile nanoHPLC sample ESI-QqTof MS (Agilent 1200 BrukerMicrOTOFQ) (ProfileAnalysis)
  • uPLC ESI-QQQ Analysis (Agilent 1260 Agilent 6410BA)

Identification and description of metabolites

  • GC/MS analysis (Thermo GC8060 MD800 EI+)
  • LC/MS/MS analysis nanoHPLC sample ESI-QqTof MS
  • Absolute and relative quantification of small molecules
  • GC/MS SIM analysis (Thermo GC8060 MD800 EI+, CI-, CI+)
  • GC FID analysis (Perkin Elmer)
  • LC/MS profile nanoHPLC sample ESI-QqTof MS (Agilent 1200 BrukerMicrOTOFQ) (ProfileAnalysis)
  • uPLC analysis ESI-QQQ , MRM (Agilent 1260 Agilent 6410BA)

Our unit has developed procedures for:

  • medium and large short-chain fatty acids in serum and cell membranes
  • amino acids
  • organic acids in urine
  • acylcarnitines in plasma
  • prostanoids in plasma
  • volatile carbon compounds in breath
  • free plasmatic thyroid hormones
  • steroid hormones in saliva
  • sugars in urine
  • mucopolysaccharides in serum and urine