Proteomics and Metabolomics

Spectrometers

Hybrid Mass Spectrometer Q-TOF Micro ESI-QqTOF MS and Nano HPLC (Agilent serie 1200™)

QqT Hybrid spectrometer coupled to a nano liquid chromotagraph for identification of peptides and proteins.  It can also carry out metabolite analysis in linear mode.   Nominal resolution of 20,000.   It is used in tandem mode to identfiy peptides in single mode for quantification for profile analyses. 

Software:

- Compass server
- HyStar 3.2
- MicrOTOF Control
- Data Analysis
- Sequence Editor
- Metabolite Detect
- Library Editor
- Quant Analysis
- Profile Analysis
Integrated with ProteinScape

MS/MS with attachment for ISD experiments (In SourceDecay)

Triple Quadruple Mass Spectrometer ESI- QQQ MS (Agilent™ 6410 BA) and uPLC quaternary (Agilent™ 1260 Infinity)

Spectrometer coupled to uPLC for metabolite quantification in MRM mode (multiple reaction monitoring).  Also operates in four other operation modes, SIM (single ion monitoring), ion precursors, ion fragments, neutral losses.

Software

- Mass Hunter Data Adquisition
- Mass Hunter QualitativeAnalysis
- Mass Hunter QuantitaveAnalysis

Gas Cromatograph coupled to Mass Spectometer

GC/EI+Ci+Ci-/MS (GC series™  con MD800™  y AS2000™  Thermo Instruments).

     – Softwares: MassLab con librerías NIST y WiLLEY

Thermal desorption for volatiles (MW-1A™ Rektorik)

Gas chromatograph FID (flame ionization)

     - GC/FID (AutoSystem™ Perkin Elmer)

Software

Clarity (6 channels)

MALDI TOF/TOF Autoflex Speed TOF/TOF

Mass spectrometer MALDI with nominal resolution for  20.000 para peptides

Capability for experiments in MS, MS/MS and three MS masses for protein molecular weights

- MS/MS with attachment for ISD (In SourceDecay)
- MS/MS with attachment LIFT peptide sequencing, glycans
- MS/MS/MS with attachment CID + LIFT for high energy fragmentation for sequencing protein terminals e fragmentación a alta energía para
 

Equipment integrated with the platform:
- Compass
- Clinprot
- ClinPro Tools 2.2
- Bio Tools 3.1
- Nano-MALDI
- WARP-LC
- MASCOT versión 2.5

Software: FlexControl, Flex Imaging, ProteinScape
Imageprep – Attachment for tissue sample preparation for MALDI imaging

Analytical and Preparative Liquid Chromatographs

HPLC quatenary pump with UV/vis Detector and Column Heater

(Waters™ 600 series)

Software

ClarityClarity (6 channels)

FPLC Äkta purifier™ (GE Healthcare)

FPLC for protein purification

Software

Unicorn

HPLC alliance™ 2695 (Waters) con detectores 2996 UV/vis y desviación del índice de refracción 2414FPLC

Software

Empower

HPLC alliance™ 2695 (Waters) con detectores 2996 UV/vis y fluorescencia 2475

Software

Empower