Microscopy

Equipment

LEICA CM1950 cryostatic microtome

Cryostatic microtome with UVC disinfection and Ag-protect system LEICA CM1950 for the manipulation of potentially dangerous specimens.   Semi-automatic with 15 plate positions, working temperature range from 18ºC to -42ºC and rapid freezing of samples to -52ºC and vacuum washing system to reduce direct manipulation of samples. 

Centrifuge system Cytofuge® 2 (StatSpin)

 Centrifuge system Cytofuge® 2 (StatSpin) for obtaining cell preparations on slides. 

Zeiss Axioskop 2 microscope

Clear field and epifluoresence Zeiss Axioskop 2 microscope with filters for the most common fluorochromes (filter sets 1, 9, 10 and 15) with 5x, 10x, 20x, 40x and 63x oil objectives coupled to a AxioCam MRC5 (D) colour camera.    ZEN blue 2011 software for obtaining and analysing images. 

Zeiss AxioObserver Z1 inverted fluorescence microscope

Zeiss AxioObserver Z1  inverted fluorescence microscopio with filter sets 1, 20 and 38HE, 10x, 20x, 40x, 40x oil and 63x oil objectives.   Coupled to AxioCam MRc5 (D) monochrome camera and ZEN blue 2012 software for obtaining and analysing images.   For medium length studies of live cells there is an external incubation chamber with a modular heating unit for 6w and 24w plaques with regulation of temperature and CO2 environment.  To capture images in optical sections there is an Apotome module to avoid light dispersion, even in thicker samples.

Confocal Zeiss LSM 710 Module

Confocal Zeiss LSM 710 module coupled to Zeiss AxioObserver Z1 microscope equipped with 7b laser beams (405, 458-488-514, 543, 594, 633); 10x, 20x, 40x, 40x oil and 63x oil objectives and ZEN black 2012 software for obtaining and analysing images.