Histopathology & Electron Microscopy

The services of the Histopathology and Electron Microscope Facility are focussed on five areas:

Histopathology Techniques

  • Mounting samples of tissues or cells from human, experimental animal or cell culture origin (preparation of the sample and selection of the area)
  • Processing samples with poor cohesion with gelatine or agar-agar
  • Impregnation of tissues with paraffin using tissue processors
  • Elaboration of paraffin blocks
  • Section cutting of frozen or paraffin-mounted histology samples
  • Histochemical staining
  • Cytological processing
  • Other histopathology techniques of the Anatomical pathology Service

Tissue Matrices

  • Search for blocks and laminas in the Anatomical pathology archives
  • Selection of areas of interest by the pathologist associated with the project
  • Tissue MicroArray (TMA) by highly qualified technician and report template to help with interpretation of the TMA

Immunohistochemistry

  • Antibody titer and technical consultation
  • Immnohistochemical staining of the antibodies habitually used in healthcare by the Anatomical pathology Service and less frequent antibodies.  These stains can be processed manually or automatically, depending on the antibody used.
  • Cutting sections of TMA and frozen tissue
  • Help with quantifying and interpreting results

Electron Microscope

  • Elaboration of resin blocks
  • Semi-fine sections stained with tolulene blue
  • Selection of adequate areas by pathologist
  • Ultra-fine sections and electron microscope staining
  • Analysis of electron microscope sections by specialist technician and digital photography
  • Expert help with interpretation from a pathologist

Molecular Biology Techniques

These techniques are those specific to the Anatomical pathology Service which works in coordination with the other facilities available at the IGTP.

  • Cutting Eppendorf or laminar sections (including dissection of the marked area by a pathologist) for different molecular biology techniques
  • Manual and Kit-mediated DNA, RNA extractions
  • Rearrangement of IGH,TCR-α, TCR-β,Ig-γ, IGH(DJ),BCL2,Cadenes Kappa,Virus HHV8.
  • Strip Assay de KRAS+BRAF and BRAF 600/601
  • Determination of the variants EGFRvIII ,BRAF(mutation V600E), IDH1,IDH1/2, GIST 2(Kit 13 i 17),GIST3 (PDGFRA 12,14,18), Kit de melanoma, NRAS by sequencing
  • Methylation of the gene Methyl Guanine Methyl Transferase (MGMT)
  • Instability of microsatellites
  • Fluorescent hybridization in situ (FISH) de BCL6, EGFR, CCND1, BCL2, MYC, SYT, EWSR1, ALK, IGH, IGK, IGL, MALT1, 1p719q, c-erbB2/HER2, IRF4, ROS1 i MET
  • New techniques as they are added to the routine of the service or relevant new techniques requested