Translational Genomics

MiSeq® Personal Sequencer (Illumina)

Massive sequencing device than can generate up to 20Gb per run, 20M reads from 2x300bp in a maximum of 384 indexed samples.  It uses sequencing by synthesis technology (SBS) and consists of amplification, sequencing and analysis of data in one instrument using the RTA v1.18.54.0 software (Real Time Analysis), MCS v2.6.1.1 (MiSeq Control Software), MSR (MiSeq Reporter) and cloud data storage (BaseSpace).

ABI Prism 3130/3130xl Genetic Analyzers (Applied Biosystems)

Sequencers with 4-16 capillaries per 96w or 383w plate, CCD detection camera and multiline argon ion laser with primary excitation in 488 and 514.5 nm. Software acquisition, Data Collection v3.1 and analysis programme Sequencing Analysis v5.4, GeneMapper v4.1 and SeqScape 2.7.

2200 TapeStation® (Agilent)

Automatic electrophoresis system which analyses nucleic acids (gDNA, DNA or RNA fragments or libraries) or proteins in a capillary support (ScreenTape) and scans images.  It allows for the calculation of the size of nucleic acids (in bp) and of proteins (kDa), quantification and quality analysis using the GeneTools software with a system for comparison of simplified bands incorporated.  The systems uses a very small sample size (1-2 µL) and readings can be done in single tubes or 96w plates.

qPCR a temps real LightCycler®480 (Roche Diagnostics)

For interchangeable plate formats 96w and 384w.   Xenon lamp, emissions 430-630 nm, filters with excitation at 440, 465, 498, 533 i 618 nm and emission at 488, 510, 580, 610, 640 i 660 nm which allow detection of the most usual fluorochromes (FAM, NED, ROX, SYBR, TAMRA i VIC). LightCycler® 480 programme for acquisition and analysis and LightCycler® Probe Design Software 2.0. 1.

Sistema Amplispeed (Beckman Coulter Biomedical GmbH)

An amplification platform for single cells, nucleic acid samples with low copy number that uses glass slides (that carry up to 48 different samples) in a final maximum volume of 1 µl.  The amplification system allows results to be read by microscope or slide fluorescence scanner.  Samples can be prepared for approximations based on flow cytometry, micromanipulation and laser microdissection and the main applications are heterogeneous and circulating tumour genetic analysis (stem cells),  PCR, rt-PCR or rt-qPCR. Preamplification of single cell for mRNA expression, amplification previous to sequencing, mitochondrial heteroplasmy studies and identification for forensic analysis.