Translational Genomics

MiSeq® Personal Sequencer (Illumina)

Massive sequencing device than can generate up to 20Gb per run, 20M reads from 2x300bp in a maximum of 384 indexed samples.  It uses sequencing by synthesis technology (SBS) and consists of amplification, sequencing and analysis of data in one instrument using the RTA v1.18.54.0 software (Real Time Analysis), MCS v2.6.1.1 (MiSeq Control Software), MSR 2.6.2.3 (MiSeq Reporter) and cloud data storage (BaseSpace).

ABI Prism 3130/3130xl Genetic Analyzers (Applied Biosystems)

Sequencers with 4-16 capillaries per 96w or 383w plate, CCD detection camera and multiline argon ion laser with primary excitation in 488 and 514.5 nm. Software acquisition, Data Collection v3.1 and analysis programme Sequencing Analysis v5.4, GeneMapper v4.1 and SeqScape 2.7.

2200 TapeStation® (Agilent)

Automatic electrophoresis system which analyses nucleic acids (gDNA, DNA or RNA fragments or libraries) or proteins in a capillary support (ScreenTape) and scans images.  It allows for the calculation of the size of nucleic acids (in bp) and of proteins (kDa), quantification and quality analysis using the GeneTools software with a system for comparison of simplified bands incorporated.  The systems uses a very small sample size (1-2 µL) and readings can be done in single tubes or 96w plates.

Real time qPCR LightCycler®480 (Roche Diagnostics)

For interchangeable plate formats 96w and 384w.   Xenon lamp, emissions 430-630 nm, filters with excitation at 440, 465, 498, 533 i 618 nm and emission at 488, 510, 580, 610, 640 i 660 nm which allow detection of the most usual fluorochromes (FAM, NED, ROX, SYBR, TAMRA i VIC). LightCycler® 480 programme for acquisition and analysis and LightCycler® Probe Design Software 2.0. 1.

NextSeqTM-1000

Massive sequencing equipment which can generate up to 120 Gb per use and 400M reads in runs of 2x150 cycles. The system uses the Illumina synthesis sequencing by synthesis technology (SBS) and can analyse results locally, by remote connection and with the secondary analysis system DRAGEN bio-IT FPGA

MINion System (Oxford Nanopore)

Massive sequencing device based on the use of nanopores suspended over a membrane with permits nucleic acids (DNA and RNA) to pass. Bases are identified by calculating the changes in electric current produced on the membrane. Ultra-long reads are generated (> 4 Mb) and up to 50 Gb of data in 72-hour runs at 420 bases/second. Suitable for whole genomes/exomes/metagenomics, directed sequencing, whole transcriptome (cDNA), small transcriptome (direct DNA) and methylation studies.

QIAxcel Advanced System

This is an automatized capillary electrophoresis system that separates fragments of DNA and RNA in function of molecular weight (up to 96 samples per run). The analysis software (BioCalculator™ software) can generate both electropherograms and images of gels and can determine: size and concentration of DNA fragments (15bp to 15Kb); b) ratio, concentration and quality of total RNA; c) cRNA/cDNA and fragmented RNA/DNA quality. The sample preparation is easy as the capillaries contain the ethidium bromide needed to visualize the fragments.

  1. Amplification takes place in a conventional thermocycler supporting a 96w plate using EvaGreen chemistry, or based on hydrolysis probes.
  2. The results of the amplification are read by a QX200 Droplet Reader. It can analyze up to 96 samples and has a fluorescence optical detection system (Multi-pixel photon) in the FAM (EvaGreen) and HEX (alternatively VIC) channels.

The device has a linear discrimination range of up to 5 orders of magnitude and a precision of ±10%.

QX200™ Droplet Digital™ PCR System

The QX200 Droplet Digital PCR (ddPCR) System is a combination of three devices:

The QX200 Droplet Generator divides samples ((20 µl) into 200 droplets using microfluidics and a oil emulsion. It can process up to a maximum of 8 samples in 5 minutes.

Robotic EpMotion 5070 Station

A robotic liquid dispensing system with two tools of one and eight channels that dispense volumes between 1 and 50µl. It can pipette volumes of 1-2µl with or without contact. It has a precision of less than 2% cv at 1µL.It has an optical sensor to detect and automatically calculate the volume of liquid, labware and tips. It is compatible with tubes (0,2 mL a 50 mL) and microplates of up to 384 wells.