The High Content Genomics and Bioinformatics

Equipment

Array scanning

HiScan (Illumina) array scanner

Confocal scanner with 0.375 micron spatial resolution  and two lasers at  532 and 660 nm wavelengths. Allows imaging and data extraction for Infinium beadarray assays to measure  fluorescent signal from probes profiling up to 5.000.000  genomic positions per sample simultaneously. Used for SNP genotyping, copy number and LOH analysis, and DNA methylation profiling.

Robotic liquid handling

Scliclone NGS (PerkinElmer) liquid handling robot

Robot with 96 disposable tip head and plate gripper. Protocols for automated  execution of molecular biology applications, especially for next generation sequencing (NGS). Allows parallel handling of up to 96 samples  simultaneously. Used for NGS library preparation and quantification and 384 microwell plate qPCR setup

Evo-150 (Tecan) liquid handling robot

Robot equiped with 8 fixed tips and set up for automated sample processing for Illumina infinium assays. Allows parallel handling of up to 384 samples. Used for SNP genotyping, copy number and LOH analysis, and DNA methylation profiling.

Sample disruption

S2 (Covaris) ultrasonicator

Ultrasound mechanical  processor based on adaptive focused acoustics. Used for genomic DNA and chromatin fragmentation, nucleic acid extraction from paraffin fixed tissue. Processes one sample at a time.