The High Content Genomics and Bioinformatics
HiScan (Illumina) array scanner
Confocal scanner with 0.375 micron spatial resolution and two lasers at 532 and 660 nm wavelengths. Allows imaging and data extraction for Infinium beadarray assays to measure fluorescent signal from probes profiling up to 5.000.000 genomic positions per sample simultaneously. Used for SNP genotyping, copy number and LOH analysis, and DNA methylation profiling.
Robotic liquid handling
Scliclone NGS (PerkinElmer) liquid handling robot
Robot with 96 disposable tip head and plate gripper. Protocols for automated execution of molecular biology applications, especially for next generation sequencing (NGS). Allows parallel handling of up to 96 samples simultaneously. Used for NGS library preparation and quantification and 384 microwell plate qPCR setup
Evo-150 (Tecan) liquid handling robot
Robot equiped with 8 fixed tips and set up for automated sample processing for Illumina infinium assays. Allows parallel handling of up to 384 samples. Used for SNP genotyping, copy number and LOH analysis, and DNA methylation profiling.
S2 (Covaris) ultrasonicator
Ultrasound mechanical processor based on adaptive focused acoustics. Used for genomic DNA and chromatin fragmentation, nucleic acid extraction from paraffin fixed tissue. Processes one sample at a time.