Germans Trias i Pujol Health Sciences Research Institute

CYTOMETRY UNIT

Flow cytometry is a technology that have arisen to enhance the capabilities of conventional microscopy. Given the strong interest in haematology, immunology and oncology of the researchers in the institute a Flow Cytometry Unit was established in 1998.

Staff
Marco Antonio Fernández Sanmartín, Facility Manager
Gerard Requena Fernández, Associate Technician.

Services
The unit works as a self‐service facility for trained users under the staff facility supervision for flow cytometric analysis. The facility personnel also perform cell sorting experiments.

Equipment

Cell sorter:

FACSAria II (Becton Dickinson). Blue, violet and red Laser with 15 parameters (FSC, SSC, FL1-13). ACDU (Automated Cell Deposition Unit), 4-way sorting, temperature control and AMO (Aerosol Management Option). NEW ADQUISITION, AUGUST 2008.
FACSVantage SE (Becton Dickinson). Laser Coherent Enterprise dual line 488/357(UV) nm. Seven parameters (FSC, SSC, FL1‐5). Options installed: MacroSort, ACDU (Automated Cell Deposition Unit). Workstation Apple Macintosh G3.
Automated immunomagnetic beads cell separator: AutoMACS (Miltenyi Biotec). Maximal load 4 x 109 cells.

Cell Analyzers:

FACScan (Becton Dickinson). Argon laser (488 nm) with 5 analysis parameters (FSC, SSC, FL1‐3). Workstation Apple Macintosh G4.
FACScalibur (Becton Dickinson). Argon (488 nm) and red diode laser (633 nm) with 6 analysis parameters (FSC, SSC, FL1‐4). Automatic Loader option installed. Workstation Apple Macintosh G4.
FACSCanto II (Becton Dickinson). Three laser (405, 488 and 635 nm) with 10 fluorescence detectors. Automatic Loader option installed. Workstation FACSDiva software version 6 (PC).

Web Site: http://citometria.germanstrias.org